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KMID : 0360220100510020276
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Journal of the Korean Ophthalmological Society
2010 Volume.51 No. 2 p.276 ~ p.281
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Effect of Glucose on the Production of Reactive Oxygen Species in Retinal Pigment Epithelial Cells
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Lee Jae-Hyung
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal pigment epithelial (RPE) cells.
Methods: ARPE-19 cells were exposed to low glucose (5 mM) and high glucose (HG, 25 mM) for three days. Additionally, 50 μM N-acetyl cysteine (NAC) and 100 μM L-arginine were co-exposed. Productions of nitric oxide (NO), ROS, and superoxide were determined by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively.
Results: HG increased production of NO, ROS, and superoxide. HG-induced cells increased production of superoxide and ROS but were suppressed by NAC and L-arginine (substrate for NO production) as an antioxidant.
Conclusions: HG increased ROS production in RPE cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in RPE cells.
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KEYWORD
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High glucose, Oxidative stress, Reactive oxygen species, Retinal pigment epithelial cells
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